A quarter of the players on the Marlins 40-man roster enter 2022 wearing different uniform numbers from the ones they used in major league games last season.
- Jesús Aguilar—switched to No. 99 (previously No. 24). Aguilar would become the first Marlins player ever to wear 99 in the regular season. He made the change to accommodate new teammate Avisaíl García.
- Anthony Bender—switched to No. 55 (previously No. 80). The last Marlin to wear 55 in the regular season was Ryne Stanek (2020).
- Edward Cabrera—switched to No.