The Under-23s clash against Stoke City at Old Trafford, which kicks off at 19:00 BST and is live on MUTV, will represent a first appearance in a United shirt for summer signing Dalot, after the defender recovered from a knee injury sustained at his former club FC Porto.
Argentinian goalkeeper Romero hasn’t played for the Reds since the 1-0 win over Watford on the final day of last season and he missed the World Cup in Russia due to a knee problem.
Fellow countryman Rojo played in the summer tournament but he didn’t play for United in pre-season after picking up an injury while on international duty.