The Reds are one of 16 academy sides that will join the 48 teams from League One and League Two in the first stage of the competition.
United will compete in Group G of the Northern Section, which comprises eight group of four teams.
The dates and times of the fixtures are yet to be decided, but each Academy team will play all their games away from home.
Mark Dempsey's youngsters will be hoping to improve on last year's campaign, where we exited at the group stage after one win (Bradford City) and two defeats (Lincoln City and Sunderland).