The 31-year-old started Saturday night's prestige friendly against Brazil at Wembley Stadium and wore the captain’s armband after regular skipper Kyle Walker was forced off early on.
Maguire played for 67 minutes against the Selecao before he was replaced by Brighton’s Lewis Dunk.
Moments later, Maguire's United team-mate Kobbie Mainoo proudly made his England debut as he entered the action alongside fellow Academy graduate Marcus Rashford.
It was confirmed by The FA earlier on Sunday evening that Harry has sustained an injury, and he will not feature in Tuesday's match against Belgium.