The centre-back has been a stalwart for his country, and produced some outstanding form in previous major tournaments.
He had hoped to recover from the calf problem that forced him onto the sidelines since the draw with Burnley in April, and ensured his absence from the Emirates FA Cup final victory against Manchester City.
However, despite linking up with Gareth Southgate's provisional squad ahead of the finals in Germany, Maguire has failed to prove his fitness in time.
"I am devastated not to have been selected to play for England at the Euros this summer," he posted on social media.