Netherlands defender Jeremie Frimpong limped out of training in tears ahead of their World Cup last-16 clash with the USA on Saturday.
Louis van Gaal's side booked their place in the knockouts with a 2-0 win Qatar on Tuesday, meaning they topped Group A.
They now face Gregg Berhalter's side at the Khalifa International Stadium at 3pm (GMT) on Saturday.
And Frimpong, who is yet to play at the tournament, with Inter Milan full-back Denzel Dumfries featuring for every minute of the group at right-wing-back, looked in pain as he hobbled away from the Organje's training session.