The competition has proved a happy hunting ground for Erik ten Hag's side in the recent past, having lifted the trophy in his first season with the Reds, claiming the crown against Newcastle United in 2023.
United enter at the third stage due to our involvement in the Europa League — with Premier League sides not competing in Europe having entered in the last round — and do so after a quick turnaround following our impressive 3-0 victory away at Southampton.
Here's everything you need to know about the upcoming fixture against the League One outfit...
HOW TO WATCH: UNITED V BARNSLEY
The Tykes' visit to Old Trafford is due to kick-off at 20:00 BST on 17 September with a place in the fourth round up for grabs.