Travis Binnion’s side - the current defending champions of the competition - beat Crystal Palace away in the previous round and are now embarking on a second consecutive away trip.
The action will kick off at 19:00 GMT at the bet365 Stadium, and it will be live on MUTV*.
As the young Reds prepare to take on the Potters in search of another positive result, here's everything you need to know...
*Live broadcasting of this fixture is available in the UK and Ireland only.
The Youth Cup is our Under-18s' first game back after the winter break, with Binnion's side beating Sunderland 1-0 five days before Christmas.