Lisandro Martinez and Noussair Mazraoui were withdrawn with around a quarter of an hour left to play at St Mary’s Stadium.
Both players were visibly limping as they left the pitch, while Matthijs de Ligt also had to be substituted.
The Dutchman, who claimed our Man of the Match award, later clarified to TNT Sports that he was suffering with cramp.
While Ten Hag said it was too early to diagnose any particular issues with the other players, he confirmed De Ligt’s condition and said his squad were still building towards full fitness at the beginning of the season.