The Manchester United defender won his 25th cap in the 3-1 victory over Poland, last time out, and is anticipating a tough test at Hampden Park in Tuesday's 19:45 BST kick-off.
Portugal are top of the group with maximum points from three matches, ahead of second-placed Croatia, with Bruno Fernandes assisting the first goal, for Manchester City's Bernardo Silva, against the Poles in Warsaw.
"It is a great pride for me to reach 25 caps for Portugal," said Dalot. "It's a very special feeling to be able to represent our country and I feel happy and fulfilled with the journey I've taken so far.