Cristian Romero and Lisandro Martinez have not been able to join their Argentina team-mates in Miami ahead of a clash with Honduras.
The Premier League centre-backs were unable to register their visas with the US Embassy in London, after it closed in the wake of Queen Elizabeth II's death, according to TyC Sports.
Romero and Martinez, who turn out for Tottenham and Manchester United respectively, will miss the opportunity to link up with their side for the last time before their World Cup squad is announced in November.
The centre-back pairing are almost certain to be included in Lionel Scaloni's final squad to make the trip to Qatar, having both impressed for their clubs already this season.