Diego Simeone confirmed in his pre-match press conference that captain Koke would miss the game with a groin injury, and the 30-year-old has not been included in Los Colchoneros' selection for the game at the Wanda Metropolitano Stadium.
Matheus Cunha and Daniel Wass are also absent through injury, as is back-up goalkeeper Benjamin Lecomte, while Yannick Carrasco is suspended.
Star forwards Antoine Griezmann, Luis Suarez and Joao Felix are all included in the list, although it is possible that only one of the trio will start the game, with Griezmann still on the injury comeback trail and Angel Correa Atleti's most in-form attacker currently.