The presentation aimed to educate the Reds' squads from the U15s to the U23s about the events of April 15, 1989 from a family perspective.
Margaret and Sue were then asked questions by the players before U23s defender Adam Lewis presented them with flowers on behalf of the club.
The Academy also hosted a special Hillsborough memorial tournament, as Liverpool played against youngsters from Nottingham Forest and Sheffield Wednesday. Sides from U9 to U14 level competed in tribute to those who lost their lives in 1989.
Academy director Alex Inglethorpe said: "To be able to welcome Margaret and Sue down to the Academy to educate the boys on the events of Hillsborough was a real honour.