The aim of the 170-mile trek – which is a continuous walk from Bradford to Liverpool via Bolton Wanderers and 15 other grounds over the weekend of September 27-29 – is to raise over £20,000 for the foundation set up by Darby and close friend Chris Rimmer.
Darby, who made six first-team appearances for Liverpool before departing in 2012 to join Bradford City, was diagnosed with motor neurone disease last year and forced to retire from football.
Former Reds Neil Mellor and Dominic Matteo are set to take part in the walk, while Steven Gerrard made a video wishing Darby and the walkers the best of luck.