Swansea City were the only development side to make it through to the quarter-finals of the EFL Trophy as four under-21 teams went out on Tuesday.
A brace from Oli McBurnie saw the Swans under-21s beat Wolves academy side 2-1 at the Liberty Stadium.
Leicester City, Reading, and Brighton's development teams all exited the competition after away losses.
Meanwhile, Luton could face a financial penalty after making seven changes in their 4-0 win against Chesterfield.
The Hatters were fined £15,000 in November for failing to field "full-strength" sides in the group stage.
Competition rules state clubs must field five players who started the previous or following game, or five who have made the most appearances this season.