A late goal from Croatia's Ivan Perisic condemned the European champions to second place in Group D and a clash with Italy in the round of 16
Spain coach Vicente del Bosque has defended his team selection following the side's 2-1 loss to Croatia at Euro 2016 on Tuesday.
Del Bosque named an unchanged line-up from Spain's first two group games - wins over Czech Republic and Turkey - despite the defending champions already booking their spot in the knockout stages.
While first place in Group D was still up for grabs, several Spain players looked fatigued in the latter stages of the match in Bordeaux, with Ivan Perisic striking an 87th minute winner.