Manchester City have included injured midfielder Rodri in their updated Champions League squad for the knockout phase while there are also places for new signings Omar Marmoush, Nico González and Abdukodir Khusanov.
Vitor Reis, who also arrived in January in a €35 million ($36.2m) deal from Palmeiras, has been omitted.
Marmoush, González and Khusanov will all be eligible for the playoff tie against Real Madrid, which begins next week.
City, who finished 22nd in the league phase table, face the LaLiga giants in the first leg at the Etihad Stadium on Tuesday.