Hull City have failed in their attempt to sign forward Yannis Salibur from French side Guingamp.
Hull agreed a reported £9m fee for the 26-year-old on deadline day but a delay in processing the paperwork meant the transfer did not go through in time.
The Tigers appealed to world governing body Fifa but have now confirmed their approach has been unsuccessful.
Hull had kept a space free on their 25-man Premier League squad list in the event the appeal went in their favour.
The Tigers nevertheless enjoyed a productive January transfer window, with head coach Marco Silva bringing in several new faces.