Chelsea Football Club will not be able play any games at Twickenham when their west London ground is being rebuilt, rugby's governing body has said.
The club will have to leave their Stamford Bridge home for up to three years during the construction of a new £500m 60,000-seat stadium.
Twickenham was an option being considered by the club but a spokesperson for the Rugby Football Union (RFU) said it would not happen.
Chelsea have declined to comment.
Wembley Stadium is thought to be another possible venue being considered by the club.