Billy Gilmour has tested positive for Covid-19 and will miss Scotland's crucial Euro 2020 match with Croatia on Tuesday.
The Chelsea midfielder was a standout as Steve Clarke's side drew 0-0 with England on Friday on his first start.
The Scottish FA currently say there are no close contacts identified from inside the camp, with 25 players fit to face Croatia.
Scotland must win at Hampden to have hope of progression from Group D.
Both sides have a point after two matches while England and the Czech Republic are on four points prior to their meeting at Wembley.