Chelsea say England left-back Ben Chilwell is expected to miss the World Cup after a scan showed he had a "significant" hamstring injury.
He has been capped 17 times by England and was in their most recent squad in September, although did not play.
England start their World Cup against Iran on 21 November, while boss Gareth Southgate names his squad on Thursday.
In a statement Chelsea said: "Following the injury sustained in our recent game against Dinamo Zagreb, Ben has undergone a scan on his hamstring.
"Results show that Ben has suffered a significant injury and the defender is unfortunately expected to miss the World Cup.