First-half goals from Ben Pearson and Jordan Hugill earned Preston a 2-0 win and extended struggling Aston Villa's winless streak to nine matches.
Pearson's early strike was added to by Hugill's tap-in as Preston jumped above Villa, who missed a late penalty through Ross McCormack, in the Sky Bet Championship table.
It was the first meeting between these Football League founder members since 1974.
Preston snatched their early lead in front of the huge travelling Villa support after just four minutes.