Newcastle's Sandro Tonali and Aston Villa's Nicolo Zaniolo have left Italy's training camp after being told they are involved in an investigation by Italian prosecutors.
The Italian football federation (FIGC) said the players were not in the right "condition" to participate in Italy's two upcoming Euro 2024 qualifiers.
The FIGC has not specified what the investigation concerns, but Italian news agency ANSA reports it relates to an illegal betting probe.
The news comes a day after FIGC prosecutors announced an investigation into 22-year-old Juventus midfielder Nicolo Fagioli, who is alleged to have used a variety of identities to place bets on illegal websites.