Ben White will not join up with England for their four Nations League games as he continues his rehab on a hamstring injury sustained at the back end of the season.
The Arsenal centre back had been called up to Gareth Southgate's latest squad but the injury sustained to his hamstring meant he has not risked his long term fitness.
Most of Southgate's original 27-man squad arrived at St George's Park on Monday as preparations got under way for the fixtures against Hungary, Germany and Italy in June.
Jarrod Bowen, who was called up to his first ever England squad for the games next month, arrived on Monday afternoon and was greeted by England staff as well as Manchester City player Jack Grealish.