Only 12 current FBS college football programs have a winning record against Alabama football. Five of those twelve teams hold an upper hand over the Crimson Tide based on a single game.
Among those 12 teams, Rice University has the most impressive undefeated record against the Crimson Tide. The Rice Owls beat the Tide three consecutive seasons 1954-1956. The 1954, 28-6 win by Rice was in the Cotton Bowl.
UCF (2000), Northern Illinois (2003), Minnesota (2004), Oklahoma State (2006) and Utah (2009 Sugar Bowl) are the teams with 1-0 records against the Alabama Crimson Tide.
Not counting the three games versus Rice and the five losses to five teams above, the team having inflicted the highest percentage of defeats on Alabama football is Texas.